Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane
Background: Hib-related meningitis and pneumonia are severe and debilitating infectious disorders that can affect anyone. The protection provided by the Haemophilus influenzae immunisation was long-lasting. Purpose of the research: The creation of a lipid nanoparticle (LNP) RNA vaccine against the genes hcsA and hcsB that allow the movement of capsular polysaccharides over the outer membrane in Haemophilus influenzae type b. Research type: Screening experimental study. Methodology: In this study, we created an LNP-RNA vaccine for Haemophilus influenzae type b hcsA and hcsB, which are involved in the pathogen's ability to transfer capsular polysaccharides through its outer membrane. Lipid nanoparticles with a particle diameter of roughly 87 nanometers made up the vaccine delivery system. Results: In preclinical experiments conducted on animals, the vaccine's efficacy was 91%, while in Phase 1/2 clinical trials, it was only about 85%. Compared to other conventional vaccines, it had superior biological activity and fewer adverse effects. Conclusion: In our investigation, the vaccination proved a promising and effective preventative measure against bacterial illness brought on by HA.
Kassab, M. (2023). Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane. International Journal of Health Sciences (Egypt), 1(3), 113-121. doi: 10.21608/ijhegy.2023.199193.1009
MLA
Mohammed M Kassab. "Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane", International Journal of Health Sciences (Egypt), 1, 3, 2023, 113-121. doi: 10.21608/ijhegy.2023.199193.1009
HARVARD
Kassab, M. (2023). 'Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane', International Journal of Health Sciences (Egypt), 1(3), pp. 113-121. doi: 10.21608/ijhegy.2023.199193.1009
VANCOUVER
Kassab, M. Design of Lipid nanoparticle (LNP)- RNA vaccine against Haemophilus influenza type b hcsA and hcsB genes facilitating the transport of capsular polysaccharides across the outer membrane. International Journal of Health Sciences (Egypt), 2023; 1(3): 113-121. doi: 10.21608/ijhegy.2023.199193.1009